The effects of ascorbic acid, auxins and sugars on somatic embryo induction of oil palm SUP-PSU were evaluated. Embryogenic callus was cultured on Y3 (Eeuwens, 1978) medium supplemented with 0.1 mg/L dicamba, 3 % sucrose and various concentrations of ascorbic acid, auxins and sugars. After culturing for 20 days, Adding 200 mg/L ascorbic acid gave the highest somatic embryo (SE) induction at 32% and number of SEs at 1.76 embryos/tube. For auxin types and concentrations, medium without plant growth regulators gave the highest globular embryo (GE) induction at 100%, number of GEs at 22.14 embryos/tube, haustorium embryo (HE) induction at 81.82% and number of HEs at 2.28 embryos/tube after 20 days of culture. In case of sugar types, 0.1 M lactose gave the highest GE induction at 100%, number of GEs at 52.68 embryos/tube, HE induction at 84.21%, number of HEs at 14.01 embryos/tube, secondary somatic embryo (SSE) induction at 52.63% and number of SSEs at 6.67 embryos/tube after 60 days of culture. Therefore, PGR-free Y3 medium with 0.1 M lactose 200 mg/L ascorbic acid and 3% sucrose was suitable for somatic embryo induction. This protocol will be used for effective in vitro propagation of oil palm SUP-PSU in the future.
The purpose of this study was to investigate effects of chitosan on in vitro seedling of Panisea uniflora (Lindl.) Lindl. on ½MS medium supplemented with different concentrations of chitosan; 0, 10, 20, 40, 60, 80, 100 and 120 mg/L for 12 weeks. The results showed that the culture media supplemented with 10 mg/L of chitosan gave the highest percentage of shoot formation (67.5%), average number of shoot (1.92 shoots/plant), average number of leaf (5.65 leaves/plant) as well as gave the high average number of root (2.58 roots/plant). P.uniflora (Lindl.) Lindl. seedlings were transferred to the greenhouse on coconut husk chops for 8 weeks gave the highest survival rate (100%), leaf formation (60%), average number of leaf (2.70 leaves/plant), average of leaf width (0.38 cm/leaf), average of leaf length (1.25 cm/leaf) including the highest percentage of root formation (43.33%), average number of root (0.57 roots/plant) difference was statistically significant (p≤0.05) compared to other planting materials.
Keywords: Panisea uniflora (Lindl.) Lindl., chitosan, growth, development, planting material
Paclobutrazol application with drenching on growing media and spraying methods using 4 concentration levels 0, 100, 300 and 500 mg/l, 3 times weekly interval for growth retardant of Guzmania “Lingulata” inflorescence was conducted at Nongnooch Pattaya Tropical Garden in Chonburi Province between February 2016 - May 2016. The application of this chemical, both drenching on growing media and spraying retarded the growth of Guzmania “Lingulata" inflorescence. 500 mg/l paclobutrazol gave reduced the inflorescence length, inflorescence diameter and primary bract length significantly as compare with control but this concentration gave too short inflorescence. However, the application of paclobutrazol had no effect on the number of day to the first flower blooming. Evaluation of Guzmania “Lingulata” quality for decoration use indicated that the Guzmania “Lingulata” which drenching with 100 mg/l paclobutrazol was the most suitable for flowering potted plant production.
Effect of panicle position at physiological maturity stage on seed quality of upland rice cv. Dawk Pa-yawm were conducted at the Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai campus, Hat Yai, Songkhla, Thailand from August 2014 to January 2015. The objective of this study was to determine seed quality of physiological maturity stage at difference panicle position. Upland rice was planted in a field and the panicles were tagged at first date of the flower started blooming to indicate the date of flowering. The panicles at physiological maturity stage (28 days after flowering) were harvested and divided into three portions; top, middle and bottom. Seeds were dried at 40 ๐C for 48 hr. to investigate seed quality. The result showed that panicle position at physiological maturity affected seed color development and seed quality. Seed from top and middle positions had higher quality than bottom position. However, all positions still had high germination and soil emergence of 98.00-99.50 and 97.00-98.00 % respectively and high vigor in terms of mean germination time of 5.20-6.58 days, seedling root and shoot length of 13.57-16.31 and 8.89-12.78 cm respectively and seedling dry weight of 7.15-9.64 mg/seedling. Thus, production of maximum quality seed of upland rice cv. Dawk Pa-yawm, the panicle should be harvested at physiological maturity when approximately 85 % of seed turned to yellow by threshing seeds from whole panicle.
Keywords: Upland Rice, Seed Quality, Physiological Maturity, Panicle Position
White leaf disease of broadleaf carpet grass (Axonopus compressus)was observed in Hatyai, Songkhla, Thailand, during 2013. To diagnose for the causative agent suspected to be a phytoplasma, 16S rDNA fragments were amplified by nested PCR with phytoplasma universal primer pair P1/P7, followed by R16F2n/R2. The PCR amplified fragments of approximately 1.2 kb were specific to the diseased plants, not present in samples from healthy plants. The fragments’ nucleotide sequences had the highest 98% identity match with Bermuda grass white leaf phytoplasma, in 16SrXIV group. This report indicates the associating of white leaf disease in broadleaf carpet grass, in Thailand, with the 16SrXIV group of phytoplasma.In addition, the broadleaf carpet grasswhite leaf phytoplasma was confirmed to be a member of ‘Candidatus Phytoplasma cynodontis’ by phylogenetic analyses.
Keywords: Broadleaf carpet grass, nested PCR, phytoplasma, white leaf disease